Effects of thymol on non-alcholic hepatic liver induced with chronic immobility in rats

Abstract Background and Aim: Considering the role of oxidative stress on non-alcoholic fatty liver disease and changing lipid profiles, the antioxidant effect of plants and fruits on biochemical indicators was investigated. Thymol is a phenolic monoterpene that has antioxidant properties. According to the above, in this study, the effects of thymol on improving the symptoms of non-alcoholic fatty liver disease caused by chronic immobility stress will be studied. Materials and Methods: In this study, 30 rats were randomly assigned to three groups according to the criteria studied and the animals. Animals tested - In this experimental study, they were divided into four control groups, stress group and stress group + thymol. The rats will be subjected to daily immobile stress for 6 hours for 60 days. After 60 days, the rats were anesthetized with ketamine and xylazine and blood was taken from the heart, and the entire liver was removed for histological examination. The rest of the liver will be stored at Kruskal–Wallis and Mann-Whitney U for further analysis and biochemistry. And the value of P<0.05 will be considered significant. Results: Our data shown that the mean of GPX and TNFα in the treatment group was significantly lower than the negative control; on the other hand, the mean of TNFα in the control group was significantly higher than the control sham. Also, showed that fibrosis of the liver tissue portal was 100% harmless in the sham control group, 25% in the negative control group (stress) without damage, and 70% isolated as single-celled necrosis and in the treatment group 5. 37% without lesions, 50% as single-celled necrosis and 12.5% ​​as necrosis. Other pathological studies have shown that lobular inflammation of liver tissue was 100% harmless in the sham control group, 25% in the negative control group (stress) without injury, 62.5% isolated as single-celled necrosis, and 12.5% Percentage occurred as necrosis and in the treatment group as 37.5% without injury, 50% as single cell necrosis and 12.5% ​​as necrosis. Findings from the previous chapter of this study showed that necrosis of liver tissue was 100% uncontrolled in the sham control group, 87.5% in the negative control group (stress) without damage and 12.5% ​​as single-celled necrosis. In the treatment group, 62.5% appeared without injury, 12.5% ​​as single-celled necrosis and 12.5% ​​as necrosis. On the other hand, malarial liver tissue, hepatocyte bolunin, liver tissue, and presinosoidal liver fibrosis were 100% harmless in all three groups: control, negative control, and treatment. Also, the results of our research showed that changes in adipose tissue adipose tissue were 100% uncontrolled in the sham control group, 12.25% in the negative control group (stress) as single-celled necrosis and 50% as necrosis in the group. Treatment was 62.5% without injury, 25% with single-celled necrosis and 12.5% ​​with necrosis. Conclusion: Studies in our research as well as research in accordance with the objectives of our study showed that thymol had a positive and significant effect on reducing TNF-α, GPX, liver tissue portal fibrosis and hepatic lobular inflammation. Therefore, it can be hypothesized that thymol can improve the function of non-alcoholic fatty liver due to chronic immobility stress.